10.26091/ESRNZ.9702404.v1
Frederic D. L. Leusch
Frederic D. L.
Leusch
Natalie H. Aneck-Hahn
Natalie H.
Aneck-Hahn
Jo-Anne E. Cavanagh
Jo-Anne E.
Cavanagh
David Du Pasquier
David Du
Pasquier
Timo Hamers
Timo
Hamers
Armelle Hebert
Armelle
Hebert
Peta A. Neale
Peta A.
Neale
Marco Scheurer
Marco
Scheurer
Steven O. Simmons
Steven O.
Simmons
Merijn Schriks
Merijn
Schriks
Comparison of in vitro and in vivo bioassays to measure thyroid hormone disrupting activity in water extracts
Institute of Environmental Science and Research
2019
In vitro
In vivo
Surface water
Thyroid activity
Wastewater
New Zealand
Environmental Science
Environmental Monitoring
Inorganic Geochemistry
2019-08-27 21:13:26
Journal contribution
https://research.esr.cri.nz/articles/journal_contribution/Comparison_of_in_vitro_and_in_vivo_bioassays_to_measure_thyroid_hormone_disrupting_activity_in_water_extracts/9702404
<p>Environmental chemicals can induce thyroid disruption
through a number of mechanisms including altered thyroid hormone biosynthesis
and transport, as well as activation and inhibition of the thyroid receptor. In
the current study six in vitro bioassays indicative of different mechanisms of
thyroid disruption and one whole animal in vivo assay were applied to 9 model
compounds and 4 different water samples (treated wastewater, surface water,
drinking water and ultra-pure lab water; both unspiked and spiked with model
compounds) to determine their ability to detect thyroid active compounds. Most
assays correctly identified and quantified the model compounds as agonists or
antagonists, with the reporter gene assays being the most sensitive. However,
the reporter gene assays did not detect significant thyroid activity in any of
the water samples, suggesting that activation or inhibition of the thyroid
hormone receptor is not a relevant mode of action for thyroid endocrine
disruptors in water. The thyroperoxidase (TPO) inhibition assay and transthyretin
(TTR) displacement assay (FITC) detected activity in the surface water and
treated wastewater samples, but more work is required to assess if this
activity is a true measure of thyroid activity or matrix interference. The
whole animal Xenopus Embryonic Thyroid Assay (XETA) detected some activity in
the unspiked surface water and treated wastewater extracts, but not in unspiked
drinking water, and appears to be a suitable assay to detect thyroid activity
in environmental waters.</p>