10.26091/ESRNZ.9560735.v1
Yao Feng
Yao
Feng
Wen-Juan Zhang
Wen-Juan
Zhang
Yuan-Wang Liu
Yuan-Wang
Liu
Jian-Ming Xue
Jian-Ming
Xue
Shuo-Qing Zhang
Shuo-Qing
Zhang
Zhao-Jun Li
Zhao-Jun
Li
A simple, sensitive, and reliable method for the simultaneous determination of multiple antibiotics in vegetables through SPE-HPLC-MS/MS
Institute of Environmental Science and Research
2019
Antibiotics
Vegetable
SPE-HPLC-MS/MS
Farming
Farmland Ecosystem
Antibiotic residues
Food safety
China
Crop and Pasture Biochemistry and Physiology
Crop and Pasture Nutrition
Farming Systems Research
2019-08-19 04:19:21
Journal contribution
https://research.esr.cri.nz/articles/journal_contribution/A_simple_sensitive_and_reliable_method_for_the_simultaneous_determination_of_multiple_antibiotics_in_vegetables_through_SPE-HPLC-MS_MS/9560735
Antibiotics, widely used in livestock breeding, enter the environment through animal manure because of incomplete absorption in animals, especially the farmland ecosystem. Therefore, antibiotics may be adsorbed by plants and even become hazardous to human health through the food chain. In this study, a simple, sensitive, and reliable method was developed for the simultaneous determination of eleven antibiotics, including four sulfonamides, two tetracyclines, three fluoroquinolones, tylosin, and chloramphenicol in different vegetable samples using SPE-HPLC-MS/MS. Vegetable samples were extracted by acetonitrile added with hydrochloric acid (125:4, <em>v</em>/<em>v</em>). The extracts were enriched by circumrotating evaporation, and then cleaned through SPE on a hydrophilic-lipophilic balance (HLB) cartridge. All compounds were determined on a C18 reverse phase column through HPLC-MS/MS. The mean recoveries of 11 antibiotics from spiked samples of vegetables ranged from 71.4% to 104.0%. The limits of detection and quantification were 0.06–1.88 μg/kg and 0.20–6.25 μg/kg, respectively. The applicability of this technique demonstrated its good selectivity, high efficiency, and convenience by the analysis of 35 vegetable samples available from a vegetable greenhouse. Antibiotic residues in vegetables have aroused wide concern from the public. Therefore, standards should be established for antibiotic residues in vegetables to ensure food safety and human health.