miRNA Signatures of Insulin Resistance in Obesity
Angela Jones
Kirsty M. Danielson
Miles C. Benton
Olivia Ziegler
Ravi Shah
Richard S. Stubbs
Saumya Das
Donia Macartney-Coxson
10.26091/ESRNZ.8061842.v1
https://research.esr.cri.nz/articles/journal_contribution/miRNA_Signatures_of_Insulin_Resistance_in_Obesity/8061842
<p><b>Objective:</b> Extracellular microRNAs (miRNAs) represent
functional biomarkers for obesity and related disorders; this study
investigated plasma miRNAs in insulin resistance phenotypes in obesity. </p>
<p><b>Methods:</b> One hundred seventy-five miRNAs were analyzed in
females with obesity (insulin sensitivity, n511; insulin resistance, n519; type
2 diabetes, n515) and without obesity (n512). Correlations between miRNA level
and clinical parameters and levels of 15 miRNAs in a murine obesity model were
investigated. </p>
<p><b>Results:</b> One hundred six miRNAs were significantly (adjusted
P[1]0.05) different
between controls and at least one obesity phenotype, including miRNAs with the
following attributes: previously reported roles in obesity and altered
circulating levels (e.g., miR-122, miR-192); known roles in obesity but no
reported changes in circulating levels (e.g., miR-378a); and no current reported
role in, or association with, obesity (e.g., miR-28-5p, miR-374b, miR-32). The
miRNAs in the latter group were found to be associated with extracellular
vesicles. Forty-eight miRNAs showed significant correlations with clinical
parameters; stepwise regression retained let-7b, miR-144-5p, miR-34a, and
miR-532-5p in a model predictive of insulin resistance (R250.57, P57.531028).
Both miR-378a and miR-122 were perturbed in metabolically relevant tissues in a
murine model of obesity. </p>
<p><b>Conclusions:</b> This study expands on the role of extracellular
miRNAs in insulin-resistant phenotypes of obesity and identifies candidate
miRNAs not previously associated with obesity.</p>
2019-05-01 02:27:23
MicroRNAs
miRNAs
Biomarkers
Obesity
Insulin resistance
Biomarkers
Epidemiology
Medical Genetics (excl. Cancer Genetics)