10.26091/ESRNZ.12237407.v1
Jo-Anne Bright
Jo-Anne
Bright
Kevin Cheng
Kevin
Cheng
Zane Kerr
Zane
Kerr
Catherine McGovern
Catherine
McGovern
Hannah Kelly
Hannah
Kelly
Tamyra R. Moretti
Tamyra R.
Moretti
Michael A. Smith
Michael A.
Smith
Frederick R. Bieber
Frederick R.
Bieber
Bruce Budowle
Bruce
Budowle
Michael D. Coble
Michael
D. Coble
Rashed Alghafri
Rashed
Alghafri
Paul Stafford Allen
Paul Stafford
Allen
Amy Barber
Amy
Barber
Vickie Beamer
Vickie
Beamer
Christina Buettner
Christina
Buettner
Melanie Russell
Melanie
Russell
Christian Gehrig
Christian
Gehrig
Tacha Hicks
Tacha
Hicks
Jessica Charak
Jessica
Charak
Kate Cheong-Wing
Kate
Cheong-Wing
Anne Ciecko
Anne
Ciecko
Christie T. Davis
Christie T.
Davis
Michael Donley
Michael
Donley
Natalie Pedersen
Natalie
Pedersen
Bill Gartside
Bill
Gartside
Dominic Granger
Dominic
Granger
MaryMargaret Greer-Ritzheimer
MaryMargaret
Greer-Ritzheimer
Erick Reisinger
Erick
Reisinger
Jarrah Kennedy
Jarrah
Kennedy
Erin Grammer
Erin
Grammer
Marla Kaplan
Marla
Kaplan
David Hansen
David
Hansen
Hans J. Larsen
Hans J.
Larsen
Alanna Laureano
Alanna
Laureano
Christina Li
Christina
Li
Eugene Lien
Eugene
Lien
Emilia Lindberg
Emilia
Lindberg
Ciara Kelly
Ciara
Kelly
Ben Mallinder
Ben
Mallinder
Simon Malsom
Simon
Malsom
Alyse Yacovone-Margetts
Alyse
Yacovone-Margetts
Andrew McWhorter
Andrew
McWhorter
Sapana M. Prajapati
Sapana M.
Prajapati
Tamara Y. Powell
Tamara
Y. Powell
Gary Shutler
Gary
Shutler
Kate Stevenson
Kate
Stevenson
April R. Stonehouse
April R.
Stonehouse
Lindsey Smith
Lindsey
Smith
Julie Murakami
Julie
Murakami
Eric Halsing
Eric
Halsing
Darren Wright
Darren
Wright
Leigh Clark
Leigh
Clark
Duncan A. Taylor
Duncan A.
Taylor
John S. Buckleton
John S.
Buckleton
STRmix™ collaborative exercise on DNA mixture interpretation (Accepted Manuscript)
Institute of Environmental Science and Research
2020
Forensic DNA Interpretation
Probabilistic Genotying
STRmix™
Inter-Laboratory Study
Intra-Laboratory Study
Forensic Biology
Genetics
2020-05-05 23:51:14
Journal contribution
https://research.esr.cri.nz/articles/journal_contribution/STRmix_collaborative_exercise_on_DNA_mixture_interpretation_Accepted_Manuscript_/12237407
An intra and inter-laboratory study using the probabilistic
genotyping (PG) software STRmix™ is reported.
Two complex mixtures from the PROVEDIt set, analysed on an Applied
Biosystems™ 3500 Series Genetic Analyzer, were selected. 174 participants responded.
<p>For Sample 1 (low template, in the order of 200 rfu for
major contributors) five participants described the comparison as inconclusive
with respect to the POI or excluded him.
Where LRs were assigned, the point estimates ranging from 2 × 104 to 8 ×
106. For Sample 2 (in the order of 2000
rfu for major contributors), LRs ranged from 2 × 1028 to 2 × 1029. Where LRs were calculated, the differences
between participants can be attributed to (from largest to smallest impact):</p>
<p>• varying
number of contributors (NoC),</p>
<p>• the
exclusion of some loci within the interpretation,</p>
<p>• differences in local CE data analysis
methods leading to variation in the peaks present and their heights in the
input files used,</p>
<p>• and
run-to-run variation due to the random sampling inherent to all MCMC-based
methods.</p>
<p>This study demonstrates a high level of repeatability and
reproducibility among the participants.
For those results that differed from the mode, the differences in LR
were almost always minor or conservative.</p>
<br>