10.26091/ESRNZ.12237407.v1 Jo-Anne Bright Jo-Anne Bright Kevin Cheng Kevin Cheng Zane Kerr Zane Kerr Catherine McGovern Catherine McGovern Hannah Kelly Hannah Kelly Tamyra R. Moretti Tamyra R. Moretti Michael A. Smith Michael A. Smith Frederick R. Bieber Frederick R. Bieber Bruce Budowle Bruce Budowle Michael D. Coble Michael D. Coble Rashed Alghafri Rashed Alghafri Paul Stafford Allen Paul Stafford Allen Amy Barber Amy Barber Vickie Beamer Vickie Beamer Christina Buettner Christina Buettner Melanie Russell Melanie Russell Christian Gehrig Christian Gehrig Tacha Hicks Tacha Hicks Jessica Charak Jessica Charak Kate Cheong-Wing Kate Cheong-Wing Anne Ciecko Anne Ciecko Christie T. Davis Christie T. Davis Michael Donley Michael Donley Natalie Pedersen Natalie Pedersen Bill Gartside Bill Gartside Dominic Granger Dominic Granger MaryMargaret Greer-Ritzheimer MaryMargaret Greer-Ritzheimer Erick Reisinger Erick Reisinger Jarrah Kennedy Jarrah Kennedy Erin Grammer Erin Grammer Marla Kaplan Marla Kaplan David Hansen David Hansen Hans J. Larsen Hans J. Larsen Alanna Laureano Alanna Laureano Christina Li Christina Li Eugene Lien Eugene Lien Emilia Lindberg Emilia Lindberg Ciara Kelly Ciara Kelly Ben Mallinder Ben Mallinder Simon Malsom Simon Malsom Alyse Yacovone-Margetts Alyse Yacovone-Margetts Andrew McWhorter Andrew McWhorter Sapana M. Prajapati Sapana M. Prajapati Tamara Y. Powell Tamara Y. Powell Gary Shutler Gary Shutler Kate Stevenson Kate Stevenson April R. Stonehouse April R. Stonehouse Lindsey Smith Lindsey Smith Julie Murakami Julie Murakami Eric Halsing Eric Halsing Darren Wright Darren Wright Leigh Clark Leigh Clark Duncan A. Taylor Duncan A. Taylor John S. Buckleton John S. Buckleton STRmix™ collaborative exercise on DNA mixture interpretation (Accepted Manuscript) Institute of Environmental Science and Research 2020 Forensic DNA Interpretation Probabilistic Genotying STRmix™ Inter-Laboratory Study Intra-Laboratory Study Forensic Biology Genetics 2020-05-05 23:51:14 Journal contribution https://research.esr.cri.nz/articles/journal_contribution/STRmix_collaborative_exercise_on_DNA_mixture_interpretation_Accepted_Manuscript_/12237407 An intra and inter-laboratory study using the probabilistic genotyping (PG) software STRmix™ is reported. Two complex mixtures from the PROVEDIt set, analysed on an Applied Biosystems™ 3500 Series Genetic Analyzer, were selected. 174 participants responded. <p>For Sample 1 (low template, in the order of 200 rfu for major contributors) five participants described the comparison as inconclusive with respect to the POI or excluded him. Where LRs were assigned, the point estimates ranging from 2 × 104 to 8 × 106. For Sample 2 (in the order of 2000 rfu for major contributors), LRs ranged from 2 × 1028 to 2 × 1029. Where LRs were calculated, the differences between participants can be attributed to (from largest to smallest impact):</p> <p>• varying number of contributors (NoC),</p> <p>• the exclusion of some loci within the interpretation,</p> <p>• differences in local CE data analysis methods leading to variation in the peaks present and their heights in the input files used,</p> <p>• and run-to-run variation due to the random sampling inherent to all MCMC-based methods.</p> <p>This study demonstrates a high level of repeatability and reproducibility among the participants. For those results that differed from the mode, the differences in LR were almost always minor or conservative.</p> <br>